Ohio Amphibians: Ecology, Identification, and their Use in AmphIBI & PHWH Assessments

The amphibian species of Ohio are an interesting group whose ecology and identification will be thoroughly covered in this course.  It will also cover how some of the species are used to assess wetlands and headwater streams.  The Amphibian Index of Biotic Integrity (AmphIBI) is a bioassessment method that uses amphibians as indicators of wetland ecological condition. Primary Headwater Habitat (PHWH) biological methods also use amphibians, specifically salamanders, to determine the quality of headwater streams.

Both the AmphIBI and the PHWH will be covered in this course, and require a firm understanding of the field protocols used to collect amphibians and the methods to make accurate identifications of amphibians to species level. Users must know how to utilize the collected data to develop wetland AmphIBI scores, assign wetland categories, and make headwater stream classification determinations. All of the information to make accurate species identifications and to determine appropriate classifications is covered in depth throughout the course.

Day One: Classroom, Laboratory and Field Instruction

The ecology of all Ohio amphibian species and the history behind the development of the AmphIBI and PHWH methods and protocols will be covered. The important morphological features that lead to identification of Ohio adult and larval amphibians will be presented in the laboratory. Each participant will have a microscope, amphibian voucher specimens, and appropriate written keys to learn the important characteristics that distinguish between adult and larval forms of species of salamanders, frogs and toads. The instructor will use a microscope that projects images on a screen to clearly indicate exactly which taxonomic features are most important to identification of the various larval forms of the species. The day will finish in the field at a local vernal pool where attendees will deploy funnel traps while learning AmphIBI field sampling protocols.

Day Two: Field Training

Participants will return to the vernal pool to gather the traps, identify and release adult amphibians, and collect and preserve larval amphibians. We will visit a headwater stream and learn the methods for collecting salamanders. Adult salamanders will be identified and released and larval salamanders will be collected and preserved.

Day Three: Laboratory and Classroom Instruction

We will be in the laboratory to identify the amphibians collected using the keys covered during Day One. Additional preserved specimens will be reviewed to make sure all participants are comfortable with distinguishing the characteristics that confirm their identification. The day will end with an explanation of the metrics that comprise the AmphIBI and how salamanders are used to classify primary headwater streams.  Participants will also learn how the data collected in the field is used to calculate AmphIBI scores and PHWH classifications. We will conclude the course with a test on the material covered.


Participants will finish the course having strong skills in identification and an understanding of the ecology of Ohio amphibians. They will also have the ability to monitor and assess vernal pools using the AmphIBI and to classify headwater streams based on their salamander communities. Upon completing the course participants will be familiar with the important principles involved in amphibian conservation and headwater stream and vernal pool protection, enhancement, management and restoration. Participants will receive a certificate documenting course completion.